Int. Rev. Allergol. Clin. Immunol. Family Med., 2016, XXII/4: 211-214 Maximize

Int. Rev. Allergol. Clin. Immunol. Family Med., 2016, XXII/4: 211-214

Title: A pilot study on house dust mites’ allergens in south-western Poland – impact of allergen-specific immunotherapy 

Authors: Królewicz E, Wolańczyk-Mędrala A, Gomułka K, Mędrala W, Barg W. 

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A pilot study on house dust mites’ allergens in south-western Poland – impact of allergen-specific immunotherapy

Królewicz E1, Wolańczyk-Mędrala A1, Gomułka K1, Mędrala W1, Barg W2.

Medical University of Wrocław, Poland: 1Department of Internal Diseases and Allergology; 2Department of Physiology

Int Rev Allergol Clin Immunol Family Med, 2016; Vol. 22, No. 4, 211

Commercially available prick tests and vaccines used for diagnosing and treatment in allergy to house dust mites comprise a mixture of naturally occurring allergens. Recognition of the allergens profile may contribute to more effective desensitization.
The aim of study
was to investigate IgE reactivity profiles against natural Dp allergen and its recombinant components rDer p 1, rDer p 2 and rDer p 10 in patients from south-west Poland who are sensitized both to Dp and Df and compare those IgE profiles of naïve patients to those who have undergone allergen-specific immunotherapy.
Materials and methods
. The study sample comprised 66 patients allergic to house dust mites (HDM) with perennial allergic rhinitis and/or asthma. Sensitization to HDM was confirmed with positive (a wheal ≥3 mm) skin prick tests (Allergopharma, Reinbek, Germany), both to Dp and Df. Thirty patients underwent allergenspecific immunotherapy (AIT) were allocated to the AIT(+) group and the remaining 36 ones to the naïve AIT(-) group. Sera from all clinically characterized patients were examined for sIgE against d1, Der p 1, Der p 2 and Der p 10 using ImmunoCAP FEIA system measurements (Pharmacia, Uppsala, Sweden) according to the manufacturer’s instructions.
. We found that 62 (94%) of the 66 analyzed patients with Dermatophagoides pteronyssinus allergy were sensitized to allergen d1. There were significant differences in IgE profiles in the naïve patients versus the ones who underwent AIT. Only 56% of AIT(-) patients were positive for rDer p 1 as compared to 83% in the AIT(+) group, p=0,0318. Similar results were found for rDer p 2: 78% patients were positive in group AIT(-) and 97% in AIT(+), p=0,0331. The most impressive discrepancy was found when simultaneous sensitization to both main allergens was taken into account. Among naïve patients only 33% responded to both allergens, while in the AIT(+) group the number was more than twice as high i.e. 80%, p=0,00040.
. Our study demonstrates the usefulness of Dermatophagoides pteronyssinus allergen d1 and its commercially available components for house dust mites’ allergy diagnosing. We proved, that in south-western Poland, IgE reactivity profile to HDM certain allergen components differed considerably between naïve patients and those who have undergone allergen-specific immunotherapy. The results of our pilot study prompt to development of research for the examination if treatment is not contributing to immunize patients with natural extracts of allergens.

Key words: Dermatophagoides pteronyssinus, Der p 1, Der p 2, Der p 10, alLergen-specific immunotherapy