Pol. Merkur. Lek (Pol. Med. J.), 2014, XXXVI/216: 379-381

SUMMARY IN POLISH & ENGLISH. FULL ARTICLE ONLY IN POLISH.

Influence of lipoic acid on the level of TNF-α in spleen homogenates

Huk-Kolega H.1, Ciejka E.1, Skibska B.2, Kowalczyk A.1, Gorąca A.1

Uniwersytet Medyczny w Łodzi: 1Katedra Fizjologii Doświadczalnej i Klinicznej, Zakład Fizjologii Krążenia, 2Katedra Farmacji Stosowanej, Zakład Farmacji Aptecznej

Lipoic acid (LA) is a natural antioxidant and possess beneficial effects on oxidative stress parameters in various tissues.
The aim of the study was to estimate of influence of LA on free -SH groups and TNF-α levels in spleen homogenates in rats during oxidative stress induced by LPS.
Material and methods. Experiments were performed on Wistar rats devided into 4 groups: group I (control) received 0,9% NaCl; group II – received LA (100 mg/kg); group III received LPS (10 mg/kg); group IV – received LPS (10 mg/kg) and 30 min later received LA at the dose 100 mg/kg.
Results. Administration of LA after LPS-induced oxidative stress resulted in statistically significant increase in the level of -SH groups and decrease in the level of TNF-α when compared to the LPS group (p< 0.001).
Conclusion. The early administration of LA after LPS challenge a significant suppressed symptoms of oxidative stress and inflammatory effects LPS, expressed as a increase in -SH groups and decrease in TNF- in the spleen homogenates.

Key words: lipoic acid, spleen, TNF-α

Pol. Merk. Lek., 2014, XXXVI, 216, 379