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Pol. Merkur. Lek (Pol. Med. J.), 2019, XLVII/281: 177-182 Maximize

Pol. Merkur. Lek (Pol. Med. J.), 2019, XLVII/281: 177-182

Title: Glutathione antioxidant system of lymphocytes in the blood of patients in a setting of concomitant chronic obstructive pulmonary disease and arterial hypertension 

Authors: Marushchak M, Maksiv K, Krynytska I, Stechyshyn I. 

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SUMMARY IN POLISH & ENGLISH. FULL ARTICLE ONLY IN ENGLISH.

Glutathione antioxidant system of lymphocytes in the blood of patients in a setting of concomitant chronic obstructive pulmonary disease and arterial hypertension

Marushchak M1, Maksiv K2, Krynytska I1, Stechyshyn I3.

1Department of Functional and Laboratory Diagnostics, I. Horbachevsky Ternopil National Medical University; 2Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University; 3Department of Pharmacy Management, Economics and Technology, I. Horbachevsky Ternopil National Medical University

The pathogenesis of both chronic obstructive pulmonary disease (COPD) and arterial hypertension (AH) is closely related to oxidative stress, which is characterized by an imbalance between the production of reactive oxygen species (ROS) and the antioxidant defense.
The aim of the study
was to assess the status of glutathione-based antioxidant protection system in lymphocytes of blood in patients with associated COPD and AH.
Materials and methods
. Lymphocytes were isolated from peripheral blood using A. Boyum technique. The quantification assay for reduced glutathione (GSH) level, glutathione reductase (GR), glutathione peroxidase (GP) and glutathione S transferase activities were assessed with spectrophotometry method.
Results
. The study population included 73 patients admitted to the Ternopil University Hospital. The subjects were distributed across three groups: group 1 (25 patients with COPD), group 2 (28 patients with COPD and AH) and the group 3 (control group of 20 healthy subjects). The analysis of parameters of lymphocytic glutathione system has not revealed any significant changes in GSH in patients with COPD only and a significant (20.5%) decrease in reduced glutathione in a COPD + AH combination. The group of patients with COPD was found to have decreased GP and GST activities, as well as increased GR activities. Patients with concomitant COPD and AH had uniform changes in the enzymes of the glutathione system. These changes were seen as significant decreases in GP activity (by 31.6%), GST activity (by 28.4%) and GR activity (by 18.8%). In patients with COPD+AH, positive correlations were found between GSH and GR/GST. In this respect, GST activity was directly correlated with GR activity and inversely correlated with GP activity.
Conclusions
. Overall, the results of our research suggest the glutathione-based antioxidant protection system to change differently in patients with COPD (GR activity increases, GP and GST activities decreases, GSH level does not change) and in patients with COPD+AH (GSH level and activities of glutathione-based antioxidant enzymes [GR, GP and GST] decreases). The changes found in the glutathione redox system suggest an important contribution of arterial hypertension to adverse course of COPD.

Key words: comorbidity, chronic obstructive pulmonary disease, arterial hypertension, glutathione system

Pol Med J, 2019; XLVII (281); 177–182