Newsletter

Pol. Merkur. Lek (Pol. Med. J.), 2015, XXXVIII/226: 203-205 Maximize

Pol. Merkur. Lek (Pol. Med. J.), 2015, XXXVIII/226: 203-205

Title: The effects of endothelin 1 and an inhibitor of phosphorylation of IκB on the content of free sulfhydryl groups (-SH) and protein in rat kidney

Authors: Gorąca A, Skibska B.  

More details

03/226

40,00 zł

SUMMARY IN POLISH & ENGLISH. FULL ARTICLE ONLY IN POLISH.

The effects of endothelin 1 and an inhibitor of phosphorylation of I
κB on the content of free sulfhydryl groups (-SH) and protein in rat kidney

Gorąca A1, Skibska B2.

Medical University of Lodz, Poland: 1Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology; 2Chair of Applied Pharmacy, Department of Pharmacy

Endothelin (ET)-1 is a potent vasoconstrictor. ET-1 an increase in the synthesis of transcription factor NF-κB, which is responsible for the production of reactive oxygen species (ROS), and cytokines.
The aim of the study was to determine whether blocking NF-κB pathway affects the content of free -SH groups and total protein in rat kidney homogenates.
Material and methods
. Experiments were carried out on four groups of male rats of Wistar-Kyoto strain. Group I – control was administered into the femoral vein solution of 0.9 % NaCl; in group II and III – was given endothelin-1 at doses of 1.5 and 12.5 μg/kg b.w., group IV – was given BAY 11-7082 (inhibitor of phosporylation of IκBα) at a dose of 10 mg/kg b.w. 30 min prior to administration of ET-1 at a dose of 12.5 μg/kg b.w.
Results
. Administration of ET-1 at doses of 1.5 and 12.5 μg/kg b.w. resulted in a statistically significant reduction of free -SH groups and protein in kidney homogenates compared to the control group. In contrast, administration of an inhibitor of nuclear factor NF-κB (BAY 11-7082 (10 mg/kg b.w.) during the oxidative stress induced by ET-1 (12.5 μg/kg b.w.) significantly increased the concentration of free -SH groups (p
Conclusions. ET-1 caused dose-dependent significant reduction of concentration free -SH groups and total protein. A inhibitor of phosphorylation of IκBα (BAY 11-7082) given during the oxidative stress induced by ET-1 (12.5 μg/kg b.w.) significantly increased the concentration of free -SH groups and protein in the kidney tissue.

Key words: endothelin 1, NF-κB blocking, oxidative stress, SH-groups

Pol Med J, 2015; XXXVIII (226); 203–205