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Pol. Merkur. Lek (Pol. Med. J.), 2012, XXXII/190: 225-227 Maximize

Pol. Merkur. Lek (Pol. Med. J.), 2012, XXXII/190: 225-227

Title: The influence of endothelin-1 and endothelin receptor blocker on the content of white blood cells in the peripheral blood of rat

Authors: Kleniewska P., Michalski Ł., Skibska B., Gorąca A.

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SUMMARY IN POLISH & ENGLISH. FULL ARTICLE ONLY IN POLISH.

The influence of endothelin-1 and endothelin receptor blocker on the content of white blood cells in the peripheral blood of rat


Kleniewska P.1, Michalski Ł.1, Skibska B.2, Gorąca A.1

Medical University of Łódź, Poland: 1Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology; 2Department of Applied Pharmacy, Department of Pharmacy

The aim of the study was to evaluate the influence of endothelin-1 (ET-1) and endothelin receptor (ETA) blocker (BQ 123) on the content of white blood cells in the peripheral blood of rat.
Material and methods
. Experiments were performed on Wistar rats divided into following groups: group I (control) received saline; group II (ET-1) received endothelin-1 (3 μg/kg b.w.); group III (BQ 123) received ETA receptor blocker (1 mg/kg b.w.); group IV (BQ 123+ET-1) received BQ 123 (1 mg/kg b.w.) and 30 minutes later ET-1 (3 μg/kg b.w.). Peripheral blood was collected from animals at three time intervals to determine the content of white blood cells using a hematology analyzer MICROS OT 45.
Results
. Administration of ET-1 resulted in a statistically significant increase in the concentration of white blood cells after 1 hour after administration, compared with the control group. Contrast, administration of a specific blocker of ETA (BQ 123) reduced the leukocyte content after 1 and 5 hours after administration of ET-1.
Conclusion
. Intravenous administration of ET-1 significantly increased the content of white blood cells in peripheral blood of rat via the ETA receptor since reversed its blockage caused the change.

Key words: endothelin-1, ETA receptor blocker, haematological parameters of blood

Pol. Merk. Lek., 2012, XXXII, 190, 225